Publications

2017

Ceramide phosphoethanolamine synthase SMSr is a target of caspase-6 during apoptotic cell death. Cabukusta B, Nettebrock NT, Kol M, Hilderink A, Tafesse FG, Holthuis JC. (2017) Biosci. Rep. 37, 4. pii: BSR20170867.

Membrane contacts, ancient and central hubs of cellular lipid logistics. Jain A, Holthuis JC. (2017) Biochim. Biophys. Acta 1864, 1450-1458

ER residency of the ceramide phosphoethanolamine synthase SMSr relies on homotypic oligomerization mediated by its SAM domain. Cabukusta B, Kol M, Kneller L, Hilderink A, Bickert A, Mina J, Korneev S, Holthuis JC. (2017) Sci. Rep. 7, 41290

Diverting CERT-mediated ceramide transport to mitochondria triggers Bax-dependent apoptosis. Jain A, Beutel O, Ebell K, Korneev S, Holthuis JC. (2017) J. Cell Sci. 130, 360-371

2016

Monitoring changes in the oligomeric state of a candidate ER ceramide sensor by single-molecule photobleaching. Cabukusta B, Köhlen JA, Richter CP, You C, Holthuis JC. (2016) J. Biol. Chem. 291, 24735-24746

Switching head group selectivity in mammalian sphingolipid biosynthesis by active-site engineering of sphingomyelin synthases. Kol M, Panatala R, Nordmann M, Swart L, van Suijlekom L, Cabukusta B, Hilderink A, Grabietz T, Mina JG, Somerharju P, Korneev S, Tafesse FG, Holthuis JC. (2016) J. Lipid Res. 57, 1273-1285

2015

Functional characterization of enzymes catalyzing ceramide phosphoethanolamine biosynthesis in mice. Bickert A, Ginkel C, Kol M, vom Dorp K, Jastrow H, Degen J, Jacobs RL, Vance DE, Winterhager E, Jiang XC, Dörmann P, Somerharju P, Holthuis JC, Willecke K. (2015) J. Lipid Res. 56, 821-835

Inner workings and biological impact of phospholipid flippases. Panatala R, Hennrich H, Holthuis JC. (2015) J. Cell Sci. 128, 2021-2032

2014

Lipid landscapes and pipelines in membrane homeostasis. Holthuis JC and Menon AK (2014) Nature 510, 48-57

Sphingomyelin synthase-related protein SMSr is a suppressor of ceramide-induced mitochondrial apoptosis. Tafesse FG, Vacaru AM, Bosma EF, Hermansson M, Jain A, Hilderink A, Somerharju P, Holthuis JC (2014) J. Cell Sci. 127, 445-454

Fat and fabulous: bifunctional lipids in the spotlight. Haberkant P and Holthuis JC (2014) Biochim. Biophys. Acta, 1841, 1022-1030

2013

In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids. Haberkant P, Raijmakers R, Wildwater M, Sachsenheimer T, Brügger B, Maeda K, Houweling M, Gavin AC, Schultz C, van Meer G, Heck AJ, Holthuis JC (2013) Angew. Chem. Int. Ed. Engl. 52, 4033-4038

Ceramide phosphoethanolamine biosynthesis in Drosophila is mediated by a unique ethanolamine phosphotransferase in the Golgi lumen. Vacaru AM, van den Dikkenberg J, Ternes P, Holthuis JC (2013) J. Biol. Chem. 288, 11520-11530

Cellular microcompartments constitute general suborganellar functional units in cells. Holthuis JC, Ungermann C. (2013) Biol. Chem. 394, 151-161

2012

Mapping functional interactions in a heteromeric phospholipid pump. Puts CF, Panatala Narendranath R, Tsavera A, Williamson P, Holthuis JC (2012) J. Biol. Chem. 287, 30529-30540

Phosphatidylserine stimulation of Drs2p-Cdc50p lipid translocase dephosphorylation is controlled by phosphatidyl-4-phosphate. Jacqout A, Montigny C, Hennrich H, Barry R, le Maire M, Jaxel C, Holthuis JC, Champeil P, Lenoir G (2012) J. Biol. Chem. 287, 13249-13261

Flip or flop: mechanism and pathophysiology of P4-ATPase-catalyzed lipid transport. Verhulst PM, Holthuis JC*, Pomorski TG* (2012) In Transmembrane dynamics of lipids, Devaux PF and Herrmann A, Eds. John Wiley & Sons, Hoboken, NJ, USA

2011

Pumping lipids with P4-ATPases. Lopez-Marques R, Holthuis JC*, Pomorski TG* (2011) Biol. Chem. 392, 67-76

2010

In vivo cross-linking of a P4-ATPase protein interaction network reveals a link between aminophospholipid transport and phosphoinositide metabolism. Puts CF, Lenoir G, Krijgsveld J, Williamson P, Holthuis JC (2010) J. Proteome Res. 9, 833-842

Cdc50 proteins are critical components of the human ATP8 subfamily of putative phospholipidtransporters. Bryde S, Hennrich H, Verhulst PM, Devaux PF, Lenoir G, Holthuis JC (2010) J. Biol. Chem. 287, 30529-30540

A brake on lipid synthesis. Tafesse FG, Holthuis JC (2010) Nature 463, 1028-1029

A flippase-independent function of ATP8B1, the protein affected in familial intrahepatic cholestasis type 1, is required for apical protein expression and microvillus formation in polarized epithelial cells. Verhulst PM, van der Velden LM, Oorschot V, van Faassen EE, Klumperman J, Houwen RHJ, Pomorski TG, Holthuis JC*, Klomp LWJ* (2010) Hepatology 51, 2049-2060

Folding defects in ATP8B1 associated with hereditary cholestasis are ameliorated by 4-phenylbutyrate. Van der Velden LM, Stapelbroek JS, Krieger E, van den Berghe, PVE, Berger R, Verhulst PM, Holthuis JC, Houwen RHJ, Klomp LW, van de Graaf SFJ (2010) Hepatology 51, 286-296

Tales and mysteries of the enigmatic sphingomyelin synthase family. Holthuis JC, Luberto C. (2010) Adv. Exp. Med. Biol. 688, 72-85

2009

Membrane contact sites between apicoplast and ER in Toxoplasma gondii revealed by Electron Tomography. Tomova C, Humbel BM, Geerts WJ, Entzeroth R, Holthuis JC, Verkleij AJ (2009) Traffic 10, 1471-1480

Sphingomyelin synthase SMS2 displays dual activity as ceramide phosphoethanolamine synthase. Ternes P, Brouwers JF, van den Dikkenberg J, Holthuis JC (2009) J. Lipid Res. 50, 2270-2277

Sphingomyelin synthase-related protein SMSr controls ceramide homeostasis in the ER. Vacaru AM, Tafesse FG, Ternes P, Kondylis V, Hermansson M, Brouwers JF, Somerharju P, Rabouille C, Holthuis JC (2009) J. Cell Biol. 185, 1013-1027

Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter Drs2p. Lenoir G, Williamson P, Puts CF, Holthuis JC (2009) J. Biol. Chem. 284, 17956-17967

Mechanism and significance of P4 ATPase-catalysed lipid transport: lessons from a Na+/K+-pump. Puts CF, Holthuis JC (2009) BBA Mol. Cell. Biol. Lipids 1791, 603-611

2007

On the origin of lipid asymmetry: the flip side of ion transport. Lenoir G, Williamson P, Holthuis JC (2007) Curr. Op. Chem. Biol. 11, 654-661

Both sphingomyelin synthase SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells. Tafesse FG, Huitema K, Hermansson M, van der Poel S, van den Dikkenberg J, Uphoff A, Somerharju P, Holthuis JC (2007) J. Biol. Chem. 282, 17537-17547

The multigenic sphingomyelin synthase family. Tafesse FG, Ternes P, Holthuis JC (2006) J. Biol. Chem. 281, 29421-29425

2006

Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles. Alder-Baerens N, Lisman Q, Luong L, Pomorski T, Holthuis JC (2006) Mol. Biol. Cell.17, 1632-1642

The CLN9 protein, a regulator of dihydroceramide synthase. Schulz A, Mousallem T, Venkataramani M, Persaud-Sawin DA, Zucker A, Luberto C, Bielawska A, Bielawski J, Holthuis JC, Jazwinski SM, Kozhaya L, Dbaibo GS and Boustany RM (2006) J. Biol. Chem. 281, 2784-2794

2005

Lipid traffic: floppy drives and a superhighway. Holthuis JC, Levine T (2005) Nat. Rev. Mol. Cell Biol. 6, 209-220

2004

Identification of a family of animal sphingomyelin synthases. Huitema K, Van Den Dikkenberg J, Brouwers JF, Holthuis JC (2004) EMBO J. 23, 33-44

The elusive flippases. Holthuis JC, Lenoir G (2004) Curr. Biol. 14, R912-913

HOR7, a multi-copy suppressor of the Ca2+-sensitive growth defect in sphingolipid mannosyltransferase-deficient yeast. Lisman Q, Urli-Stam D, Holthuis JCM (2004) J. Biol. Chem. 279, 36390-36396

Tracking down lipid flippases and their biological functions. Pomorski T, Holthuis JC, Herrmann A, Van Meer G (2004) J. Cell Sci. 117, 805-13

Protein sorting in the late Golgi of Saccharomyces cerevisiae does not require mannosylated sphingolipids. Lisman Q, Pomorski T, Vogelzangs C, Urli-Stam D, de Cocq van Delwijnen W, Holthuis JC (2004) J. Biol. Chem. 279, 1020-1029

2003

Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis. Pomorski T, Lombardi R, Riezman H, Devaux PF, van Meer G, Holthuis JC (2003) Mol. Biol. Cell 14, 1240-1254

Sensing membrane curvature. Holthuis JC, Burger KN (2003) Dev. Cell 5, 821-822

Lipid microdomains, lipid translocation and the organization of intracellular membrane transport. Holthuis JC, van Meer G, Huitema K (2003) Mol. Membr. Biol. 20, 231-241

2001

The organising potential of sphingolipids in intracellular membrane transport. Holthuis JC, Pomorski T, Raggers RJ, Sprong H, van Meer G (2001) Physiol. Rev. 81, 1689-1723

2000

Lipid Traffic: The ABC of transbilayer lipid movement. Raggers RJ, Pomorski T, Holthuis JC, Kälin N, van Meer G (2000) Traffic 1, 226-234

Inhibition of the vacuolar H+-ATPase perturbs the transport, sorting, processing and release of regulated secretory proteins. Schoonderwoert VTG, Holthuis JC, Tanaka S, Tooze SA, Martens GJM (2000) Eur. J. Biochem. 267, 5646-5654

Sphingolipid transport in eukaryotic cells. Van Meer G, Holthuis JC (2000) BBA Mol. Cell. Biol. Lipids 1486, 145-170

1999-1996

Biosynthesis of the vacuolar H+-ATPase accessory subunit Ac45 in Xenopus pituitary. Holthuis JC, Jansen EJ, Schoonderwoert VT, Burbach JP, Martens GJM (1999) Eur. J. Biochem. 262, 484-491

The syntaxin Tlg1p mediates trafficking of chitin synthase III to polarized growth sites in yeast. Holthuis JC, Nichols BJ, Pelham HRB (1998) Mol. Biol. Cell 9, 3383-3397

Two syntaxin homologs in the TGN/endosomal system of yeast. Holthuis JC, Nichols BJ, Dhruvakumar S, Pelham HRB (1998) EMBO J. 17, 113-126

The Sec1p homologue Vps45p binds to the syntaxin Tlg2p. Nichols BJ, Holthuis JC, Pelham HRB (1998) Eur. J. Cell Biol. 77, 263-268

Intracellular trafficking of the vacuolar H+-ATPase accessory subunit Ac45. Jansen EJR, Holthuis JC, McGrouther C, Burbach JP, Martens GJM (1998) J. Cell Sci. 111, 2999-3006

Secretogranin III is a sulfated protein undergoing proteolytic processing in the regulated secretory pathway. Holthuis JC, Jansen EJR, and Martens GJM (1996) J. Biol. Chem. 271, 17755-17760

The neuroendocrine proteins secretogranin II and III are regionally conserved and coordinately expressed with proopiomelanocortin in Xenopus intermediate pituitary. Holthuis JC, Martens GJM (1996) J. Neurochem. 66, 2248-2256

1995-1989

Molecular probing of the secretory pathway in peptide hormone-producing cells. Holthuis JC, Jansen EJR, van Riel MHCM, Martens GJM (1995) J. Cell Sci. 108, 3295-3305

Translocon-associated protein TRAPd and a novel TRAP-like protein are coordinately expres­sed with proopio-melanocortin in Xenopus intermediate pituitary. Holthuis JC, van Riel MHCM, Martens GJM (1995) Biochem. J. 312, 205-213

A vertebrate homolog of the actin-bundling protein fascin. Holthuis JC, Schoonderwoert VTG, Martens GJM (1994) BBA Gene Struct. Expr. 1219, 184-188

The activation of blood coagulation factor X on the surface of endothelial cells: a comparison with various vascular cells, platelets and monocytes. Brinkman HJM, Mertens K, Holthuis JC, Zwart-Huinink LA, Grijm K, van Mourik JA (1994) Brit. J. Haematol. 87, 332-342

Prevention of side effects by hemoglobin solutions: the selection of optimal testmodels, especially concerning thrombogenity. Bakker JC, Bleeker WK, Den Boer PJ, Biessels PTM, Achterberg J, Rigter G, Holthuis JC, Mertens K (1994) Artif. Cells Blood Substit. Immobil. Biotech. 22, 577-585

Coordination of protein-DNA interactions in the promoters of human H4, H3, and H1 histone genes during the cell cycle, tumorigenesis, and development. Van Wijnen AJ, Owen TA, Holthuis JC, Lian JB, Stein JL, Stein GS (1991) J. Cell. Physiol. 148, 174-189

Tumor cells exhibit deregulation of the cell cycle histone gene promoter factor HiNF-D. Holthuis JC, Owen TA, van Wijnen AJ, Wright KL, Ramsey-Ewing A, Kennedy MB, Carter R, Cosenza SC, Soprano KJ, Lian JB, Stein JL, Stein GS (1990). Science 247, 1454-1457

Modifications of protein-DNA interactions in the proximal promoter of a cell-growth-related histone gene during onset and progression of osteoblast differenti­ation. Owen TA, Holthuis JC, Markose E, van Wijnen AJ, Wolfe SA, Grimes SR, Lian JB, Stein GS (1990) Proc. Natl. Acad. Sci. USA 87, 5129-5133

Nerve growth factor-induced changes in the intracellular localization of the Protein Kinase C substrate B-50 in pheochromocy­toma PC12 cells. Van Hooff CAM, Holthuis JC, Oestreicher AB, Boonstra J, de Graan PNE, Gispen WH (1989) J. Cell Biol. 108:1115-1125

Production of insulin-like growth factors, platelet-derived growth factor, and transforming growth factors and their role in the density-dependent growth regulation of a differentiated embryonal carcinoma cell line. Van Zoelen EJJ, Koornneef I, Holthuis JC, Ward-van Oostwaard ThMJ, Feijen A, de Poorter TL, Mummery CL, van Buul-Offers SC (1989) Endocrinol. 124, 2029-2041